t75 cell culture flask (Sarstedt)

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Sarstedt t75 cell culture flask
T75 Cell Culture Flask, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t75+cell+culture+flasks/flasks+t75/pmc13054839-207-9-13
Average 86 stars, based on 1 article reviews

t75 cell culture flask - by Bioz Stars, 2026-09

86/100 stars

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Article Title: 3D Differentiation of Bone‐Marrow Derived Mesenchymal Stromal Cells into the Keratocyte Lineage for Corneal Bioprinting
Article Snippet: Human Stromal Fibroblasts (HSF) were kindly provided by Aachen University Clinic cornea bank and were cultivated in DMEM/F12 supplemented with 5% FBS, 1% Pen/Strep, and 1% Amphotericin B (15290018, Gibco, Germany). .. Two surface conditions of T75 cell culture flasks (83.3911.002, Sarstedt, Germany) were utilized: uncoated (standard tissue culture plastic) and collagen‐coated. .. Collagen (type I from calf skin in HCl; L7213, Sigma–Aldrich, Germany) was applied at a density of 10 μg cm − 2 by incubating each flask with 9.4 mL of an 80 μg mL −1 coating solution, prepared by diluting a 4 mg mL −1 stock solution in sterile 1X phosphate‐buffered saline (PBS).

Article Title: Putative α7-selective ligands interact with α9-containing nicotinic acetylcholine receptors and modulate immune functions of human mononuclear phagocytes
Article Snippet: .. For generating THP-1 cell-derived M1-like macrophages, 6 x 10 6 cells were seeded in T75 cell culture flasks (Sarstedt, Nümbrecht, Germany) and incubated with 50 nM PMA in complete medium (10% FBS) for 24 h, followed by 24 h resting in fresh complete medium without PMA. ..

Article Title: 3D Differentiation of Bone-Marrow Derived Mesenchymal Stromal Cells into the Keratocyte Lineage for Corneal Bioprinting.
Article Snippet: Human Stromal Fibroblasts (HSF) were kindly provided by Aachen University Clinic cornea bank and were cultivated in DMEM/F12 supplemented with 5% FBS, 1% Pen/Strep, and 1% Amphotericin B (15290018, Gibco, Germany). .. Two surface conditions of T75 cell culture flasks (83.3911.002, Sarstedt, Germany) were utilized: uncoated (standard tissue culture plastic) and collagen-coated. .. Collagen (type I from calf skin in HCl; L7213, Sigma– Aldrich, Germany) was applied at a density of 10 μg cm−2 by incubating each flask with 9.4 mL of an 80 μg mL−1 coating solution, prepared by diluting a 4 mg mL−1 stock solution in sterile 1X phosphate-buffered saline (PBS).

Article Title: Antioxidant potential of tree bark extracts: Insight from the multi-level output of the Antioxidant Power 1 assay
Article Snippet: The medium was supplemented with 2 mM stable L-glutamine (Capricorn, Ebsdorfergrund, Germany), 1% Penicillin-Streptomycin (10,000 units penicillin and 10 mg streptomycin/ml) (Sigma-Aldrich, St. Louis, MO, USA) and 10% FBS (Capricorn, Ebsdorfergrund, Germany) and is referred to as growth medium. .. HaCaT cells were maintained at 37 °C in a humidified atmosphere with 5% CO 2 and split once a week to varying cell densities in T75 cell culture flasks (Sarstedt, Nümbrecht, Germany). .. For splitting or seeding, the old medium was discarded, cells were washed with phosphate buffered saline (PBS; Gibco life technologies, Carlsbad, CA, USA) and detached utilizing TrypLE Express (Gibco life technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Article Title: Conjugation frequency of ESBL- and pAmpC- E. coli in broiler chickens in vivo and in vitro.
Article Snippet: .. Bacterial conjugation in intestinal chicken cells and cell-free media Intestinal chicken cells CHIC Clone-8E11 ((CHIC) Tentamus Pharma & Med Deutschland GmbH) were grown in T75 cell culture flasks (Sarstedt, Nümbrecht, Germany) and maintained in DMEM/F-12 medium (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 20% fetal bovine serum. .. For conjugation assay, cells were seeded in 12-well cell culture plates (standard surface, flat base; Sarstedt, Nümbrecht, Germany) at a density sufficient to reach 75% confluence.

Article Title: Antioxidant potential of tree bark extracts: Insight from the multi-level output of the Antioxidant Power 1 assay.
Article Snippet: The medium was supplemented with 2 mM stable L-glutamine (Capricorn, Ebsdorfergrund, Germany), 1% PLOS One | https://doi.org/10.1371/journal.pone.0328790 July 28, 2025 6 / 16 Penicillin-Streptomycin (10,000 units penicillin and 10 mg streptomycin/ml) (Sigma-Aldrich, St. Louis, MO, USA) and 10% FBS (Capricorn, Ebsdorfergrund, Germany) and is referred to as growth medium. .. HaCaT cells were maintained at 37 °C in a humidified atmosphere with 5% CO 2 and split once a week to varying cell densities in T75 cell culture flasks (Sarstedt, Nümbrecht, Germany). .. For splitting or seeding, the old medium was discarded, cells were washed with phosphate buffered saline (PBS; Gibco life technologies, Carlsbad, CA, USA) and detached utilizing TrypLE Express (Gibco life technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Article Title: Conjugation frequency of ESBL- and pAmpC- E. coli in broiler chickens in vivo and in vitro
Article Snippet: .. Intestinal chicken cells CHIC Clone-8E11 ((CHIC) Tentamus Pharma & Med Deutschland GmbH) were grown in T75 cell culture flasks (Sarstedt, Nümbrecht, Germany) and maintained in DMEM/F-12 medium (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 20% fetal bovine serum. .. For conjugation assay, cells were seeded in 12-well cell culture plates (standard surface, flat base; Sarstedt, Nümbrecht, Germany) at a density sufficient to reach 75% confluence.

Article Title: Reliable and Sensitive Detection of Carbonylated Proteins by Oxime Blot
Article Snippet: .. T75 cell culture flasks (Sarstedt AG & Co. KG, catalog number: 83.3911.002) 14. .. Mini Trans-Blot ® filter paper (Bio-Rad, catalog number: 1703932) 15.

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